大孔吸附树脂纯化甘草酸的研究

Study on Purification of Glycyrrhizic Acid with Macroporous Adsorption Resin

目的:探讨大孔吸附树脂AB-8对甘草酸的吸附性能及其影响因素。方法:采用高效液相色谱法和正交试验优选AB-8树脂的最佳精制条件。结果:以流速1ml/min,pH6.4～7.4,原液浓度为10 mg/ml的组合上柱,甘草酸的精制效果最好。结论:AB-8树脂对甘草酸的吸附量大,洗脱容易,分离效果好,产品纯度可达95％以上。

Objective:To study the adsorption and desorption behavior of macroporous adsorbption resin AB-8 for glycyrrhizic acid and its effect factors. Methods: The optimum purification conditions were investigated by high performance liquid chromatography and the orthogonal experiments. Results:The optimum conditions were as follows:the flow rate at 1 ml/min,pH at 6.4 ~7. 4 and the solution concentration at 10 mg/ml. Conclusion; AB-8 had a high adsorption capacity for glycyrrizic acid and elution was easy.