抗除草剂基因导入四倍体菘蓝

Transformation of Bar Gene to Tetraploid of Isatis indigotica

以CaMV 35S为启动子,将带有抗除草剂基因(bar基因)的pCAMBIA 3300植物双元表达载体,导入根癌农杆菌菌株EHA 105,作为基因工程菌转化四倍体菘蓝,选出5mg/L的草丁膦(ppt)为适宜的筛选压。筛选得到的抗性植株经PCR鉴定,结果表明bar基因已转移到四倍体菘蓝的基因组,从而建立了农杆菌介导的四倍体菘蓝的遗传转化系统,为利用基因工程进一步改良菘蓝品质奠定了基础。

The transgenic tetraploid of Isatis indigotica mediated by Agrobacterium tumefaciens was obtained. To transfer the plant binary expression vector pCAMBIA 3300 carrying bar gene, the Agrobacterium tumefaciens strain EHA 105 was used as engineering bacterium. The results of PCR indicated that the bar gene had been transferred into and merged with the genome of Isatis indigotica. This study will make foundation for improvement of other characters of this species with genetic engineering.