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Androgen-independent growth in LNCaP cell lines and steroid uridine diphosphate-glucuronosyltransferase expression 被引量：1

Androgen-independent growth in LNCaP cell lines and steroid uridine diphosphate-glucuronosyltransferase expression
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摘要 Aim: To investigate the mechanism of androgen-independent growth of prostate cancer after androgen ablation in LNCaP cells and the effect of glucuronidation activity. Methods: To establish androgen-independent growth in prostate cancer LNCaP-SF, continuous passage was performed in androgen-stripped medium and the cells were evaluated for glucuronidation activity. The expression vector of antisense uridine diphosphate glucuronosyltransferase (UGT) 2B15 cDNA was also constructed and evaluated. Results: LNCaP-SF lead to a higher expression in UGT2B15 and their glucuronidation activity is 2.5 times higher than that of LNCaP cells. Significantly fewer LNCaP and LNCaP-SF than control were transfected with the antisense UGT2B15 cDNA, suggesting that UGT2B15 plays an important part in the glucuronidation activity of androgens in both cells. Conclusion: The alteration of UGT2B15 expression in LNCaP-SF cells is proposed as a biological characteristic involved in the growth of hormonerefractory prostate cancer. Aim: To investigate the mechanism of androgen-independent growth of prostate cancer after androgen ablation in LNCaP cells and the effect of glucuronidation activity. Methods: To establish androgen-independent growth in prostate cancer LNCaP-SF, continuous passage was performed in androgen-stripped medium and the cells were evaluated for glucuronidation activity. The expression vector of antisense uridine diphosphate glucuronosyltransferase (UGT) 2B15 cDNA was also constructed and evaluated. Results: LNCaP-SF lead to a higher expression in UGT2B15 and their glucuronidation activity is 2.5 times higher than that of LNCaP cells. Significantly fewer LNCaP and LNCaP-SF than control were transfected with the antisense UGT2B15 cDNA, suggesting that UGT2B15 plays an important part in the glucuronidation activity of androgens in both cells. Conclusion: The alteration of UGT2B15 expression in LNCaP-SF cells is proposed as a biological characteristic involved in the growth of hormonerefractory prostate cancer.
作者 Jiro Kanaya Mitsuhiro Takashima Eitetsu Koh Mikio Namiki
机构地区 Department of Intrgrated Cancer Therapy and Urology Kanazawa University Graduate School of Medical Science
出处 《Asian Journal of Andrology》 SCIE CAS CSCD 2003年第1期9-13,共5页 亚洲男性学杂志(英文版)
关键词 LNCAP GLUCURONIDATION UGT2B15 antisense cDNA PCDNA3.1 LNCaP glucuronidation UGT2B15 antisense cDNA pcDNA3.1
分类号 Q57 [生物学—生物化学]
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参考文献16

  • 1Guillemette C. Glucuronosyltransferase activity in human cancer cell line LNCaP. Mol Cell Endocrinol 1995; 107:131-9.
  • 2Hum DW, Belanger A, Levesque E, Barbier O, Beaulieu M,Albert C, et al. Characterization of UDP-glucuronosyl-transferases active on steroid hormones. J Steroid Biochem Mol Biol 1999: 69: 413-23.
  • 3Levesque E, Beaulieu M, Guillemette C, Hum DW, Belanger A. Effect of interleukins on UGT2B 15 and UGT2B 17 steroid uridine diphosphate-glucuronosyltransferase expression and activity in the LNCaP cell line. Endocrinology 1998; 139: 2375-81.
  • 4Belanger A, Hum DW, Beaulieu M, Levesque E, Guillemette C, Tchemof A, et al. Characterization and regulation of UDPglucuronosyltransferases in steroid target tissues. J Steroid Biochem Mol Biol 1998; 65: 301-10.
  • 5Guillemette C, Hum DW, Belanger A. Specificity of glucuronosyltransferase activity in the human cancer cell line LNCaP,evidence for the presence of at least. J Steroid Biochem Mol Biol 1995; 55: 355-62.
  • 6Belanger A, Guillemette C, Hum DW. Evidence for a role of glucuronosyltransferase in the regulation of androgen action in the human prostatic cancer cell line LNCaP. J Steroid Biochem Mol Biol 1996; 57: 225-31.
  • 7Koh E, Kanaya J, Namiki M. Adrenal steroids in human prostatic cancer cell lines. Arch Andro1 2001; 46:117-25.
  • 8Kokontis JM, Hay N, Liao S. Progression of LNCaP prostate tumor cells during androgen deprivation: hormone-independent growth, repression of proliferation by androgen, and role for p27Kip 1 in androgen-induced cell cycle arrest. Mol Endocrinol 1998; 12: 941-53.
  • 9Gao M, Ossowski L, Ferrari AC. Activation of Rb and decline in androgen receptor protein precede retinoic acid-induced apoptosis in androgen-dependent LNCaP cells and their androgen-independent derivative. J Cell Physiol 1999; 179: 336-46.
  • 10Culig Z, Hoffmann J, Erdel M, Eder IE, Hobisch A, et al.Switch from antagonist to agonist of the androgen receptor bicalutamide is associated with prostate tumour progression in a new model system. Br J Cancer 1999; 81: 242-51.

同被引文献12

  • 1Hsing AW,Devesa SS,Jin F,Gao YT.Rising incidence of prostate cancer in Shanghai,China[].Cancer Epidemiology Biomarkers and Prevention.1998
  • 2Kuwano K,Hagimoto N,Nomoto Y,Kawasaki M,Kunitake R,Fujita M,et al.P53 and p21 (Wafl/Cipl) mRNA expression associated with DNA damage and repair in acute immune complex alveolitis in mice[].Laboratory Investigation.1997
  • 3McKenna NJ,Lanz RB,O‘Malley BW.Nuclear receptor coregulators:cellular and molecular biology[].Endocrine Reviews.1999
  • 4Choi YH,Lee WH,Park KY,Zhang L.p53-independent induction of p21 (WAF1/CIP1) ,reduction of cyclin B1 and G2/M arrest by the isoflavone genistein in human prostate carcinoma cells[].Japanese Journal of Cancer Research.2000
  • 5Herranz Amo F,Arias Funez F,Arrizabalaga Moreno M,Calahorra Fernandez FJ,Carballido Rodriguez J,Diz Rodriguez R,et al.The prostate cancer in the community of Madrid in 2000 I.-Incidence[].Actas Urologicas Espanolas.2003
  • 6Sarma AV,Schottenfeld D.Prostate cancer incidence,mortality,and survival trends in the United States:1981-2001[].Seminars in Urologic Oncology.2002
  • 7Kokontis JM,Hay N,Liao S.Progression of LNCaP prostate tumor cells during androgen deprivation:hormone-independent growth,repression of proliferation by androgen,and role for p27Kip1 in androgen-induced cell cycle arrest[].Journal of Molecular Endocrinology.1998
  • 8Eder IE,Haag P,Basik M,Mousses S,Bektic J,Bartsch G,et al.Gene expression changes following androgen receptor elimination in LNCaP prostate cancer cells[].Molecular Carcinogenesis.2003
  • 9Rouault JP,Prevot D,Berthet C,Birot AM,Billaud M,Magaud JP,et al.Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1,the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex[].Journal of Biological Chemistry.1998
  • 10Rouault JP,Rimokh R,Tessa C,Paranhos G,Ffrench M,Duret L,et al.BTG1,a member of a new family of antiproliferative genes[].EMBO Journal.1992

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Asian Journal of Andrology
2003年 第1期

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