邻氨基苯甲醚对中国仓鼠肺细胞毒性作用的实验研究

An Experimental Study on Toxic Effect of o-Anisidine on LungCells of Chinese Hamster

目的 观察邻氨基苯甲醚对中国仓鼠肺细胞 (Chinesehamsterlungcells ,CHL)的毒性作用。方法 噻唑蓝 (MTT)比色法。结果 不同浓度的邻氨基苯甲醚 (6 86 ,13 71,2 7 4 3,5 4 85 ,10 9 70 μg/ml 培养液 )处理一定时间后 ,细胞存活率均下降 ,呈现出时间 -剂量 -反应关系 ,同时显微镜观察 ,细胞形态发生改变。结论 邻氨基苯甲醚可以抑制CHL细胞生长 ,降低线粒体代谢活性。

Objective To observe the toxic effect of o anisidine on Chinese hamster lung cells.Methods MTT colorimetric assay was used.Results After Chinese hamster lung cells were treated with different concentrations of o anisidine culture solution (6 86,13 71,27 43,54 85,and 109 70 μg/ml respectively) for some time,the survival rate decreased with a dose time dependent effect relationship.Morphological changes were also observed.Conclusions o Anisidine could inhibit the proliferation of Chinese hamster lung cells and decrease the metabolic activity of mitochondria.