用新试剂2-QADNm在羟乙基阳离子表面活性剂存在下测定微量锌

4-(2-Quinolylazo)-1,3-dihydroxynaphthalene as a new photometric reagent for trace zinc determination

在稀碱溶液中,当有羟乙基阳离子表面活性剂 DEMAC 存在时,Zn^(2+)与2-QADNm 形成1:2的红色配合物。其λ_(max)为530nm,ε为1.75×10~5L·mol^(-1)·cm^(-1)。锌含量在0.013～1.30μg/mL 范围内服从比尔定律。用本法测定人发和血清中的微量锌,结果满意。

The azo compound 4-(2-quinolylazo)-1,3-dihydroxynaphthalene(2-QADNm)has been usedas a new sensitive spectrophotometric reagent forthe determination of trace amount of zinc.In a0.024mol/L sodium hydroxide solution containing0.030% DEMAC(dodecyl(2-hydroxyethyl)-dimethyl ammonium chloride),zinc(Ⅱ)formswith 2-QADNm a 1:2 red complex,the molarabsorptivity being 1.75×10~5 L·mol^(-1)·cm^(-1)at 530nm.Beer's law is obeyed over the range of 0.013to 1.3 ppm of zinc.A spectrophotometric methodfor the determination of zinc has been workedout,which has been applied to the determinationof zinc in human hair and blood samples.