重症急性胰腺炎微循环障碍的实验研究

Experimental study on microcirculatory disorder in severe acute pancreatitis

目的 :探讨急性出血性坏死性胰腺炎杂种犬胰腺血循环障碍的机制。方法 :用 5 %牛黄胆酸钠溶液胰管注入 ,制备急性出血性坏死性胰腺炎模型。动态观察犬和血管紧张素转化酶两血管活性物质的变化与血浆中血栓素A2 /前列腺素I2 、内皮素 /一氧化氮胰腺血流量的内在联系。结果 :急性出血性坏死性胰腺炎犬胰腺血流量明显持续下降 ,血管紧张素转化酶、血栓素A2 、前列腺素I2 、一氧化氮显著升高 ,且血栓素A2 /前列腺素2 和内皮素 /一氧化氮比值持续增加。结论 :血管紧张素转化酶、血栓素A2 /前列腺素I2 和内皮素 /一氧化氮是介导急性出血性坏死性胰腺炎犬胰腺微循环障碍的重要因素 ,出现急性出血性坏死性胰腺炎时胰腺微循环强烈收缩和微小血栓形成 。

Objective To study the mechanism of microcirculatory disorder in dogs with severe acute pancreatitis.Methods The dog model of severe acute pancreatitis was estabished by injecting 5% Sodium taurocholate into the bile-pancreatic duct.The immanent relationship was observed between pancreatic blood flow,angiotension-converting enzyme and plasma thromboxane A 2/prostaglandin I 2 and endothelin/nitric oxide.Results During severe acute pancreatitis,the pancreatic blood flow was reduced significantly and continousy,but the plasma levels of angiotension-converting enzyme,plasma thromboxane A 2,prostaglandin I 2,endothelin,nitric oxide,and ratio of plasma thromboxane A 2/prostaglandin I 2 and endothelin/nitric oxide were progressively increased.Conclusion The increase of angiotension-converting enzyme,plasma thromboxane A 2,prostaglandin I 2,endothelin,nitric oxide levels and T/P and endothelin/nitric/oxide ratio in the plasma play key roles in the pathologic changes of severee acute pancreatitis in dogs.In severe acute pancreatitis,pancreatic microcirculatory disorder of pancreas.