摘要
目的 探讨雷公藤甲素 (triptolide ,TL)对培养的大鼠气道平滑肌细胞 (ASMC)增生及原癌基因c fos与c jun表达的影响。方法 按不同药物分为 6组 ,TL组 (浓度分别为 5 ,2 0 ,80 ,32 0 ,12 80 μg·L-1)及地塞米松 1mg·L-1组 ,采用MTT法检测不同浓度药物对ASMC增殖的影响 ,同时进行逆转录 聚合酶链反应 (RT PCR)检测各组c fos ,c jun的mRNA表达水平。 结果 MTT方法显示TL对ASMC增殖有明显抑制作用 ,并呈剂量依赖性 ,TL的IC50 为 5 1.5 2mg·L-1;RT PCR方法显示TL浓度分别为 80 ,32 0 ,12 80 μg·L-1和地塞米松 1mg·L-1能显著抑制c fos ,c junmRNA表达。 结论 TL有抑制ASMC增生的作用 ,其机制可能与下调c fos和c jun表达有关。
OBJECTIVE: To investigate the effects of triptolide on airway smooth muscle cells (ASMC) proliferation and expressions of oncogene c-fos and c-jun in culturing ASMC. METHODS: The primary ASMC from rat were collected and cultured and studied. The effects of triptolide at different doses (5,20,80,320 and 1 280 μg·L-1) and dexamethasone (1 mg·L-1) on ASMC proliferation were studied by MTT method. The mRNA expressions of c-fos and c-jun form ASMC were examined by RT-PCR. RESULTS: The ASMC proliferation was inhibited at the dose (IC50 = 51.52 mg·L-1). The mRNA expression of c-fos and c-jun in ASMC was also inhibited by triptolide (80,320 and 1 280 μg·L-1)and dexamethasone. CONCLUSIONS: It suggested that triptolide remarkably inhibited ASMC proliferation probably by suppressing the expression of c-fos and c-jun.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2003年第5期348-350,共3页
Chinese Pharmaceutical Journal
基金
广东省高教厅课题 (973 5 )
