前列泌尿栓定性定量方法的研究

Studies on the identification and quantitation of Qianlie Miniao suppository

目的 研究前列泌尿栓质量控制方法 ,制定其质量标准。方法 采用薄层色谱法对前列泌尿栓 2种药物进行定性鉴别 ,采用高效液相色谱法测定黄柏中盐酸小檗碱和血竭中血竭素的含量。结果 2种药物薄层定性鉴别重现性好 ,斑点圆整 ;盐酸小檗碱和血竭素平均回收率分别为 98.83% (RSD为 1.6 9% )和 98.17% (RSD为 1.0 6 % )。结论 本方法专属性强 ,结果准确 ,检验快速 。

OBJECTIVE: To establish a specific assay for Qianlie Miniao suppository. METHOD: TLC was used to make a qualitative determination for 2 drugs in the formulary. The contents of berberine and dracorhobin were determined by HPLC. RESULTS: 2 drugs in the formulary were identified with good repetition. The average recovery of berberine and dracorhobin were 98.83% (RSD 1.69%) and 98.17% (RSD 1.06%) respectively. CONCLUSION: The method was sensitive, accurate and rapid. It was helpful to establish specification for Qianlie Miniao suppository.