高效毛细管电泳法测定人血清中美罗培南浓度

Determination of meropenem in human serum by high performance capillary electrophoresis

目的 建立测定人血清中美罗培南浓度的高效毛细管电泳方法。方法 血清样品用乙腈沉淀蛋白。以头孢哌酮为内标 ,运行缓冲液为 6 6mmol·L-1磷酸盐缓冲液 ( pH =5 .8) ;正极压力进样 ,进样压力 5kPa× 10s ;分离电压 30kV ,柱温 2 5℃ ,检测波长 2 97nm。结果 美罗培南及内标与血清中其他内源性杂质完全分离 ,在 5～ 2 0 0mg·L-1范围内美罗培南 /内标的峰面积比与浓度呈良好的线性关系 ;方法回收率 98.4 5 %～ 10 1.4 2 % ;日内和日间测定RSD分别小于 8%和 14 % ;最低检测浓度为4mg·L-1。结论 本方法简便、快速、准确 。

OBJECTIVE: To establish a HPCE method for the determination of meropenem in human serum. METHODS: The serum samples were processed with acetonitrile to denature the protein. With cefoperazone as the internal standard, the running buffer solution was 66 mmol&middotL-1 phosphate buffer (pH = 5.8). The constant voltage was 30 kV and detection wavelength was 297 nm. RESULTS: The linearity between concentrations and peak area ratio was obtained from 5-200 mg&middotL-1. The average recoveries were 98.45%-101.42%. The within-day and between-day RSD were less than 8% and 14%, respectively. The detection limits were 4 mg&middotL-1. CONCLUSION: The method was simple, fast and accurate. It is suitable for clinical pharmacokinetic study.