摘要
通过借鉴植物DNA的CTAB提取方法,结合总RNA的LiCl沉淀法,摸索出一套适合于棉花组织总RNA提取和纯化的技术—CTAB 酸酚法。该方法与异硫氰酸胍法或冷酚法等相比具有更简便、得到的棉花组织总RNA完整性好和纯度高等优点。
Based on the CTAB extraction method for cotton total DNA and the LiCl precipitation method of RNA, a new method suitable for cotton total RNA extraction, named CTAB acidic pheonlic method, was come into being. The method could produce cotton total RNA with higher purity and integrality in a shorter time from different tissues of cotton than other conventional methods.
出处
《棉花学报》
CSCD
北大核心
2003年第3期166-167,共2页
Cotton Science
基金
国家重大基础研究发展规划项目(2002CB111301)