利用CTAB/酸酚法提取棉花组织总RNA

Extraction of Total RNA in Cotton Tissues with CTAB-acidic Phenolic Method

通过借鉴植物DNA的CTAB提取方法,结合总RNA的LiCl沉淀法,摸索出一套适合于棉花组织总RNA提取和纯化的技术—CTAB 酸酚法。该方法与异硫氰酸胍法或冷酚法等相比具有更简便、得到的棉花组织总RNA完整性好和纯度高等优点。

Based on the CTAB extraction method for cotton total DNA and the LiCl precipitation method of RNA, a new method suitable for cotton total RNA extraction, named CTAB acidic pheonlic method, was come into being. The method could produce cotton total RNA with higher purity and integrality in a shorter time from different tissues of cotton than other conventional methods.