慢性牙周炎患者中间普氏菌临床分离株中溶血基因的分布

DISTRIBUTION OF THE HEMOLYTIC GENES IN PREVOTELLA INTERMEDIA ISOLATED STRAINS FROM CHRONIC ADULT PERIODONTITIS

目的 :研究溶血基因在慢性成人牙周炎患者中间普氏菌临床分离株中的分布状况 ,探讨溶血基因与中间普氏菌致病性的关系。方法 :收集符合纳入标准的 5 7例慢性成人牙周炎病人的牙周袋内的龈下菌斑 ,采用厌氧培养技术分纯产黑色素的革兰氏阴性厌氧杆菌 ,用 16SrRNA聚合酶链反应 (PCR)方法鉴定为中间普氏菌的菌株 ,保存并用溶血基因的相应引物检测该基因。结果 :5 7例慢性成人牙周炎患者中 ,2 0例检出中间普氏菌 ,检出率为 35 1%。 35株中间普氏菌的临床分离株中 ,5株能够检测到溶血基因 ,另外 30株不能检测到溶血基因。结论

Objective:To investigate the distribution of the hemolytic genes in Prevotella intermedia(Pi)isolated strains from chronic adult periodontits and explore the relations between the hemolytic genes and periodontits.Methods:Subgingival plaques were collected from 57 patients with chronic adult periodontitis and cultured in an aerobic condition.Black pigmented gram negative anaerobes were picked out and identified for prevotella intermedia using the PCR assay.The strains identified as prevotella intermedia were kept and detected for hemolytic genes using the PCR assay.Results: Of the 57 patients,20 patients were detected of prevotella intermedia.The prevalence of Pi in chronic adult periodontitis was 35 1%.Of the 35 prevotella intermedia isolates,5 strains were detected of hemolytic genes,and the other 30 strains were not detected of hemolytic genes.Hemolytic genes showed polymorphism distribution in Prevotella intermedia isolates.Conclusion: The hemolytic genes in prevotella interwedia isolates show polymorphism distribution.