黄芪对兔周围神经再灌注损伤的保护作用

Protective effect of astragalus on peripheral nerve ischemia reperfusion injury in rabbist

目的 探讨黄芪注射液对家兔周围神经缺血再灌注损伤的影响。方法 40只家兔建立周围神经再灌注模型 ,随机分成 5组 :对照组、缺血组、再灌注组、黄芪治疗Ⅰ组、黄芪治疗Ⅱ组 ,动态观察坐骨神经相应各项指标变化。结果 随制成血再灌注时间延长 ,缺血组、再灌注组运动神经传导速度分别减慢为 ( 4 .10 2± 0 .992 )m/s、( 5 .898± 8.891)m /s ,动作电位波幅分别降低为( 4 .2 71± 1.0 71)mV、( 2 .2 5 2± 1.5 2 7)mV ,潜伏期延长为 ( 1.92 1± 0 .2 0 4)ms、( 2 .3 93± 0 .2 3 1)ms ;组织超氧化物歧化酶 (SOD)活性降低分别为 ( 18.93± 9.88) μU/L、( 2 8.13± 9.72 ) μU /L、脂质过氧化产物丙二醛 (MDA)含量增加 ( 1.2 9± 0 .2 1) μmol/L、( 4 .2 9± 0 .14 ) μmol/L(P <0 .0 5 ) ,病理改变加重。黄芪Ⅰ组相对缺血组、黄芪Ⅱ组相对再灌注组神经传导速度增快 ( 3 .182± 0 .83 9)ms、( 3 .2 71± 1.0 13 )ms ,动作电位波幅增高 ( 1.867± 0 .5 12 )mV、( 2 .467± 0 .2 19)mV ,潜伏期缩短( 1.3 17± 0 .3 2 4)ms、( 2 .3 87± 0 .175 )ms ;组织SOD活性增加 ( 10 .64± 0 .79) μU/L、( 13 .94± 0 .83 )μU/L ,脂质过氧化产物MDA含量降低 ( 1.69± 0 .0 1)mol/L、( 1.0 6± 0 .2 0 )mol/L ( P

Objective To study the effect of Astragalus on peripheral nerve ischemia reperfusion injury in rabbits.Methods Models of unilateral xciatic nerve ischemia were produced in 40 rabbits.They were randomly divided into 5 groups: control group,ischemia group,reperfusion group,Astragalus-treated group Ⅰand Astragalus-treated group Ⅱ.The changes in the parameters of sciatic nerve were observed.Results With the prolongation of the duration of ischemia and reperfusion,in the ischemia group and reperfusion group,the nerve conductive velocity was slown down to (4.101±0.992) and (5.898± 8.891) m/s,the action potential amptitude decreased to (4.271±1.071) and (2.252±1.527) mV,the latent time prolonged to (1.921±0.204) and (2.393±0.231) ms,the contents of the malondialdehyde (MDA) was increased to (1.29±0.21) and (4.29±0.14) μmol/L and superoxide dismutase (SOD) was declined to (18.93±9.88) and (28.13±9.72) μU/L,respectively ( P <0.05,or P<0.001).As compared with ischemia group by Astragalus-treated group I or with reperfusion group by Astragalus-treated II,the nerve conductive velocity was speeded up [(3.182±0.839) ms,(3.271±1.013) ms],the action potential amptitude elevated [(1.867±0.512) mV,(2.467±0.219) mV],the latent time shortened [(1.317±0.324) ms,(2.387±0.175) ms],SOD activity increased [(10.64±0.79) μU/L,(13.94±0.83) μU/L],MDA decreased [(1.69±0.01) μmol/L,(1.06±0.20) μmol/L],respectively (P<0.05 orP<0.001).The pathological changes were alleviated.Conclusion Astragalus has protective effects against ischemia and reperfusion injury of peripheral nerve in rabbits.