激肽系统在ED大鼠模型阴茎组织中的表达变化研究

The expression of kallikrein-kinin system in penile tissue of ED rat model

目的研究激肽释放酶-激肽系统在ED大鼠模型中的表达变化,探讨其生物学意义。方法具有正常性功能的雄性SD大鼠120只,其中正常对照组(N组)20只,余100只为造模组(M组),采用环境雌激素样饮食联合冷应激干预条件建立复合应激大鼠模型,通过性行为学实验和阿朴吗啡(APO)阴茎勃起实验筛选出ED模型(ED组),未成ED者为复合应激组(S组)。从ED组和S组中分别抽取20只大鼠分为ED用药组(ED-Y组)和应激用药组(S-Y组),用药组以伊木萨克片干预,每日1次,每次250mg/kg,2周后取材,利用逆转录聚合酶链式反应(RT-PCR)、Western-blot技术和免疫组化方法检测大鼠阴茎组织中激肽释放酶1、T-激肽原mRNA及蛋白表达水平。结果激肽释放酶1在S组与ED组均显著低于N组,经药物分别干预后,S-Y组与ED-Y组均较S组与ED组组显著升高,P<0.05;T-激肽原在S组与ED组显著高于N组,分别经药物干预后均显著高于S组与ED组,P<0.05。结论 (1)激肽系统改变参与了ED的发生发展,并可能在ED产生中发挥着重要作用;(2)伊木萨克片治疗ED的机制与调控激肽系统有关。

Objective To study the expression of kallikrein-kinin system in rats with impotence syndrome and discussionon its biological functions. Methods 120 male SD rats with normal sexual, 20 rats were selected as the control group(N group), and 100 rats as the model group(M group). The ED rat model was screened by sexual behavior experiment after establishing the model ofcompound stress by environmental estrogen-like diet combined with cold stress. M group was divided into stress model(S group, without ED),pharmacological intervention group after stress(S-Y group), ED model group(ED group), pharmacological intervention group after ED(ED-Y group). The rats in the drug group was treated with 250 mg/kg of Yimusake for 2 weeks. The expressions of kallikrein1, T-kininogen in penile tissues were detected by RTPCR, western-blot and immunohistochemistry. Results The expression of kallikrein1 in S and ED group was significantly decreaded compared with the N group and restored after pharmacological intervention. Expression of T-kininogen S and ED group was significantly increased compared with the N group and restored after pharmacological intervention. Conclusion (1) The changes ofkallikrein-kinin system may participate in the occurrence and development of ED.(2) Yimusake is effective in the treatment of ED, its mechanism may be associated with regulation of kallikrein-kinin system.