新疆地区MTHFR基因C677T多态性与男性严重少精子症的相关性分析

Association between C677T polymorphism of MTHFR gene and severe oligozoospermia in Xinjiang

目的研究分析新疆地区亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与男性严重少精子症的相关性分析。方法选取我院生殖助孕中心与泌尿男科就诊的中重度严重少精子症汉族患者154名为病例组,以同一时间段就诊我院经精液分析且精液质量正常的男性为对照组。比较两组MTHFR C677T基因多态性的分布及频率的差异有无统计学差异。结果病例组与对照组比较,TT基因型及等位基因分布及频率均有差异(P<0.05)。病例组中CC型基因频率(21.4%)明显低于对照组(<32.3%),而病例组TT型基因频率(42.3%)明显高于对照组(>20.8%),两者进行比较,结果显示差异具有统计学意义(P<0.05;OR=2.926,95% CI=1.721～4.975),在基因型为CT的病例组与对照组的比较,其差异不具有统计学意义(P>0.05)。两组等位基因T进行比较,差异具有统计学意义(P<0.05;OR=1.847,95% CI=1.396～2.443)。结论 MTHFR基因的TT型基因及T等位基因可能是严重少精子症的危险因素。

Objective To study the relationship between the C677T polymorphism of MTHFR gene and severe oligospermia in Xinjiang. Methods 154 cases of moderate to severe oligospermia were selected as the experiment group from the reproductive assistant pregnancy center and the urology andrology department of our hospital. The male patients with normal semen parameters were set as the control group at the same time. The distribution and frequency of MTHFR C677T gene polymorphism were comparatively analyzed between the two groups(P <0.05 as the standard). Results The distribution and frequency of the TT genotype and allele in the experiment group were significantly different from those in the control group(P <0.05). The frequency of CC genotype in the experiment group(21.4%) was remarkably lower than that in the control group(< 32.3%) and that of the TT genotype in the experiment group(42.3%) was obviously higher than that in the control group(> 20.8%). The results showed that the difference was statistically significant(P < 0.05;OR = 2.926;95% CI = 1.721-4.975). In genotype CT, there was no significant difference between case group and control group(P >0.05). The alleles T of the two groups were compared, and the difference was statistically significant(P <0.05;OR =1.847, 95% CI =1.396-2.443). Conclusion The TT gene and the T allele of MTHFR gene may be a risk factor for severe oligozoospermia.