牛磺酸对急性肺损伤时内皮细胞的保护作用研究

The protective effect of Taurine on acute lung injury

目的探讨牛磺酸对大鼠急性肺损伤的保护作用及其相关机制。方法将66只大鼠分成3组,即正常对照组(n=6只)、肺损伤组、牛磺酸组(n=30只)。静脉注射脂多糖5mg/kg,建立急性肺损伤模型,腹腔注射牛磺酸50 mg/kg干预,于注射后3、6、9、12、24 h时间点采集标本,分别进行肺湿干比重测定、血气分析、肺组织病理学观察,并采用ELISA法分别检测血浆及肺泡灌洗液中的血管性血友病因子(vWF)的水平。结果肺湿干比重在肺损伤组显著升高,牛磺酸治疗组降低;动脉氧分压水平在肺损伤各组中较对照组明显降低,牛磺酸治疗组有所提高;肺损伤组病理组织切片显示炎症,肺泡间隔明显增厚,肺泡腔变窄;牛磺酸组使上述病理变化改善。vWF在各肺损伤组中较对照组均有所升高,在牛磺酸治疗组降低。结论牛磺酸干预能使肺组织病理变化较肺损伤组减轻,血浆及肺泡内vWF降低,提示牛磺酸对肺血管内皮细胞具有保护作用。

Objective To investigate the protective effect by taurine on acute lung injury and its relative mechanism.Methods 66rats were randomly divided into three groups: normal control group(n=6),lung injury group(n=30) and the Taurine group(n=30).Acute lung injury model was established by injection of lipopolysaccharide(LPS) 5 mg/kg.And the Taurine 50mg/kg were intraperitoneally injected and then specimens were collected at 3 h,6 h,9 h,12 h,24 h time point after injection,respectively.Measurement of lung wet-dry weight,blood gas,lung histopathology were observed and analyzed.von Willebrand factor(vWF) levels were detected in plasma andbronchoalveolar lavage fluid by ELISA.Results Lung wet-dry weight showed a significantly increased in lung injury group and a decreased in certain degrees in Taurine group.Arterial oxygen levels in the lung injury group were significantly lower than the control group,but increased in Taurine group.Lung injury group showed marked inflammation with marked thickening of alveolar septa and alveolar space narrowing.Improved the pathological changes were observed in the Taurine group.vWF were increased in lung injury group than in the control group.But after the intervention,they were lower in Taurine treatment group.Conclusion Intervention of Taurine could make injury in lung tissue biopsy significantly reduced compared with the LPS group.And vWF is significantly lower and therefore it prompts that Tautine had a protective effect on pulmonary vascular endothelial cells.