苦参碱对异丙肾上腺素致心肌缺血大鼠机体抗氧化酶的影响

Effects of matrine on antioxidant parameters in rats with isoproterenol-induced myocardial ischemia injury

目的研究苦参碱对异丙肾上腺素致心肌缺血大鼠机体抗氧化酶的影响。方法将80只SD大鼠分为对照组、模型组,给药10 d分为50、100、200 mg·kg-1组,给药2 d分为50、100、200 mg·kg-1组,应用分光光度计法检测大鼠血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)的活性和丙二醛(MDA)含量。结果与正常对照组比较,模型组大鼠血清中MDA含量增高,SOD、CAT、GSH-PX活力均下降(P<0.05);与模型组比较,给药10 d(50、100、200 mg·kg-1·d-1)组和给药2 d(100、200 mg·kg-1·d-1)组大鼠血清中MDA的含量降低,SOD、CAT、GSH-PX活力增强,差异有统计学意义(P<0.05),但给药2 d 50 mg·kg-1组作用效果不佳,其SOD、MDA和GSH-PX在大鼠血清中的含量较模型组差异均无统计学意义(P>0.05)。结论苦参碱能够增强内源性抗氧自由基酶的活性,进而减轻自由基对心肌组织的过氧化损伤。

Objective To study the effects of matrine on antioxidant parameters in plasma of rats with isoproterenol-induced myocardial ischemia injury. Methods Sprague-Dawley rats were divided into eight groups: control,isoproterenol( 85 mg · kg-1),acute matrine( 50,100 and 200 mg·kg-1),chronic matrine( 50 mg·kg-1,100 mg·kg-1and 200 mg·kg-1). The activity of SOD,GSH-PX,CAT and concentration of MDA were examined by spectrometer. Results After acute oral administration of matrine( 100 or200 mg·kg-1per day for 2 days respectively) and chronic oral administration of matrine( 50,100 or 200 mg·kg-1per day for 10 days respectively),the activity of SOD,CAT,GSH-PX increased greatly,and the MDA concentration reduced significantly( P < 0. 05);There was no significant changes in acute oral administration of matrine( P > 0. 05). Conclusion Matrine can improve the activity of antioxidants and relieve myocardial ischemia injury