E-选择素S128R基因多态性与复发性阿弗他溃疡关系的研究

Relationship between E-selection S128R gene polymorphism and susceptibility of recurrent aphthous ulcer

目的研究E-选择素(E-selectin)S128R基因多态性与复发性阿弗他溃疡的关系。方法选择129例复发性阿弗他溃疡患者和135例正常对照组,采用限制性片段长度多态性-聚合酶链式反应(RFLP-PCR)法对E-选择素S128R位点的基因多态性的检测进行分析,用比值比(OR)和95%可信区间(95%CI)估计相对危险度。结果 E-选择素S128R位点在基因型频率与等位基因频率的分布上,病例组与正常对照组之间差异有统计学意义(P<0.05)。E-选择素第4外显子S128R位点基因型SR(OR=4.701,95%CI=2.273~9.723)与RR(OR=5.591,95%CI=0.614~50.880)为高风险基因型,R等位基因为高风险等位基因(OR=4.177,95%CI=2.195~7.947)。结论 E-选择素第4外显子S128R位点基因多态性与复发性阿弗他溃疡有一定的相关性;E-选择素S128R位点是RAU患者的易感基因位点;E-选择素S128R位点携带R等位基因患RAU的风险性是携带S等位基因者的4.177倍。

Objective To explore the possible relationship between recurrent aphthous ulcer(RAU)and E-selectin S128 R gene polymorphism.Methods A total of 129 RAU patients were recruited for this study.One hundred and thirty-five subjects were in the normal control group.E-selectin S128 R sites were detected by restriction fragment length polymorphism-polymerase chain reaction(RFLP-PCR).Relative risk ratios were estimated by odds ratios(OR)and 95%confidence interval(95%CI).Results Significant differences were found in the genotype frequencies or allele frequencies of E-selectin S128 R sites between the RAU patients and controls(P<0.05).SR genotype(OR=4.701,95%CI=2.273~9.723),RR genotype(OR=5.591,95%CI=0.614~50.880),and R allele(OR=4.177,95%CI=2.195~7.947)at the E-selectin S128 R site exhibited high risks.Conclusion E-selectin S128 R is associated with ROU.E-selectin S128 R sites are associated with the risk of ROU and he E-selectin S128 R R allele may serve as genetic determinants for RAU.