迈氏唇鞭毛虫的培养观察

Observation on External Culture of Chilomastix mesmili

目的 :研究迈氏唇鞭毛虫在体外培养的生长情况。方法 :采用 L ocke- Egg- Serum(L ES)培养基 ,血清浓度为 2 5 % ,PH5 .8和 6 .8,无菌条件下 37℃培养。结果 :滋养体能在 L ES培养基中生长 ,接种第 2天后 ,生长量达最大。转种培养时 ,生长情况没有初始培养生长旺盛。结论 :迈氏唇鞭毛虫的体外培养 ,在目前情况下 ,初始培养能获取更多的虫体。

Objective: To study the growth of Chilomastix mesmili outside the host. Methods: Using the Locke-Egg-Serum(LES) culture medium, blood serum consistence is 25%, pH5.8 and pH6.8, culturing at 37 degree centigrade at asepsis. Result: The trophozoites can develop in LES well. On the second day after inoculating, the Chilomastix mesmilies growth at most quantity. At transferring culture, the growth of Chilomastix mesmilies is not better than at first culture. Conclusions: The external culture of Chilomastix mesmili can get more trophozoites at first culture than at transferring culture.