凝结芽孢杆菌对感染肠炎沙门氏菌蛋鸡肠道形态、抗氧化能力及沙门氏菌定植的影响

Effects of dietary supplementation with Bacillus coagulans on the intestinal morphology,plasma antioxidation capacity and colonization of Salmonellain the caecum of S.enteritidis infected laying hens

为研究凝结芽孢杆菌对感染肠炎沙门氏菌蛋鸡肠道形态、抗氧化能力及沙门氏菌定植的影响,将400只沙门氏菌阴性蛋鸡随机分成4组,每2组分在环境条件相同的2个安全隔离舍,每组设5次重复,每个重复20只鸡。对照组(I组和III组)饲喂基础饲粮,试验组(II组和IV组)在基础饲粮上添加2.5×1010cfu/kg凝结芽孢杆菌。饲喂1周后,试验组连续两天定量口服沙门氏菌菌液,对照组口服等量无菌PBS溶液,饲养3周。结果表明:1)日粮添加凝结芽孢杆菌极显著增加十二指肠绒毛宽度和绒毛吸收表面积(PBC <0.01);添加凝结芽孢杆菌可显著增加空肠绒毛长度和绒毛长度/隐窝深度比值(PBC<0.05)。沙门氏菌极显著降低空肠隐窝深度(PSE <0.01),显著降低绒毛宽度(PSE<0.05),极显著增加绒毛长度/隐窝深度比值(PSE<0.01)。2)日粮添加凝结芽孢杆菌后,沙门氏菌与凝结芽孢杆菌对血浆SOD有显著交互作用(PSE×BC <0.05),沙门氏菌可以显著增加血浆SOD和TAOC(PSE<0.05);添加凝结芽孢杆菌显著降低血浆中SOD和MDA的含量(PBC<0.05)。3)在攻毒后第1天、第14天和第21天,日粮添加凝结芽孢杆菌可显著降低盲肠沙门氏菌拷贝数(PBC<0.05);在整个试验过程中观察发现,凝结芽孢杆菌可显著降低盲肠沙门氏菌拷贝数(PBC <0.05)。综上,产蛋后期蛋鸡感染肠炎沙门氏菌后,日粮添加凝结芽孢杆菌可改善肠道形态,提升其抗氧化能力,降低盲肠沙门氏菌定植数量,缓解病源性感染应激,保证了蛋鸡机体健康。

This study evaluated the effects of Bacillus coagulans (BC) on the intestinal morphology, plasma antioxidation capacity, pro-inflammatory cytokines and colonization of caecum salmonella in S. enteritidis (SE) infected laying hens. A total of 400 Salmonella-negative, healthy hens were randomly divided into 4 treatment groups with 100 in each group, among which every two groups were allotted to same biosafety isolated sheds. Each group consisted of 5 replicates with 20 hens per replicate. Different dietary treatments were:I, basal diet;Ⅱ, basal diet + oral Salmonella administration;Ⅲ, basal diet +2.5x1010 CFU/kg B. coagulans, IV, basal diet+2.5x1010 CFU/kg B. coagulans +oral Salmonella administration. All hens in Ⅲ and IV groups were orally challenged with Salmonella at day 7 and day 8 after the initiation of experiment, whereas hens in other two groups were treated with the same amount of sterile PBS. The results showed that:1) B. coagulans significantly increased (PBC<0.05) the duodenum villus width and villus surface area. Meanwhile, B. coagulans significantly increased (PBC<0.05) the jejunum villus height and VH:CD ratio. However, Salmonella significantly decreased (PSE<0.01) the jejunum crypt depth, villus width and VH:CD ratio (PSE<0.05). 2) B. coagulans significantly decreased (PBC<0.05) the plasma of superoxide dismutase (SOD) activity and malondialdehyde (MDA). Salmonella significantly increased (PSE<0.05) SOD activity and total antioxidant capacity (T-AOC). 3) During the post-SE-challenge period, B. coagulans significantly reduced (PEF<0.05) Salmonella copies at day 1, 14 and 21 post inoculation (dpi). Meanwhile, from dpi 1 to dpi 21, the Salmonella copies in layers fed with B. coagulans was lower than (PEF<0.05) hens fed with basal diet. It is concluded that B. coagulans can improve intestinal health, alleviate the oxidative stress caused by Salmonella infection, reduce the colonization of Salmonella in caecum, relieve the stress of pathogenic infection and promote the health of birds.