紫背菜离体培养及植株再生

Plant Regeneration in Vitro Culture of Gynura bicolor D.C

以紫背菜茎段为外植体,通过不定芽诱导及生根培养,获得了再生植株;以无菌苗根茎叶为外植体,研究愈伤组织的诱导与分化。结果表明:茎段诱导不定芽培养基以MS+6-BA1.0mg/L+NAA0.2mg/L+3.0%蔗糖为佳。试管苗生根培养在1/2MS添加NAA浓度0～1.2mg/L范围内均可,但NAA浓度增加时,诱导生根时间可缩短。3种外植体均可成功诱导愈伤组织,以根外植体诱导率最高,培养基为MS+6-BA0.3mg/L+2,4-D1.0mg/L+4.5%蔗糖。愈伤组织分化培养仅得到不定根。

With Gynura bicolor D. C stems as explants,the regenerated plants were obtained by adventitious buds inducing and rooting. Callus induction and differentiation were studied using roots,stems and leaves from aseptic seedlings as explants. The results showed the optimal medium for adventitious buds induction of stems is MS+6-BA1. 0 mg/L + NAA0. 2 mg/L + 3.0% sugar. The test-tube plantlet can root in the medium 1/2MS+0～1. 2 mg/L NAA,and the rooting time is shorter with a higher concentration of NAA. Callus were induced from three kinds of explants and inductivity of root explants is the highest in medium MS+6- BA0. 3 mg/L+2,4- D 1. 0 mg/L + 4. 5% sugar. Only roots were differentiated from callus.