香蕉皮果胶纤维素酶法提取工艺

Cellulose Enzymatic Extraction Technology of Pectin from Banana Peel

研究了纤维素酶法提取香蕉皮果胶的最佳工艺。以香蕉皮果胶提取率为指标,通过单因素试验和正交试验,研究在pH值为4.5时,加酶量、液料比、提取温度和提取时间对果胶提取率的影响。结果表明:纤维素酶法提取香蕉皮果胶的最佳工艺为加酶量0.2%、液料比4mL/g、提取时间3h和温度40℃,在此条件下,香蕉皮中果胶提取率为9.33mg/g鲜香蕉皮。

The optimum process of pectin extraction from banana peel with the aid of enzyme cellulose was studied.The effect of enzyme loading,liquid to solid ratio,extraction duration and temperature on pectin extraction yield were optimized by using single factor and orthogonal(L9(3)4)test.The maximum yield of the pectin(9.33 mg/g fresh weight)was obtained when enzyme loading,liquid to solid ratio,extraction duration and temperature were 0.2%,4 mL/g,3 h and 40 ℃,respectively.