高效液相色谱法测定辣椒类调味制品中苏丹红、罗丹明B和胭脂红的方法

Determination of Sudan,Rhodamine B and Carmine in Chili Products by HPLC

制定了高效液相色谱法(HPLC)测定辣椒类调味制品中苏丹红、罗丹明B和胭脂红的检测方法。待测样品以便捷的前处理方式,通过C18反相色谱柱,使其在二极管阵列检测器上逐一分离出来。结果表明,6种检测物色谱峰形独立完整,各自分离,线性良好且基线平缓,回收率在90.7%~104.0%,相对标准偏差1.35%~4.75%。该法因试样提取程序简单快速,检测结果准确灵敏,可适用于辣椒及其制品中6种工业检测物的同时测定。

A method for determining Sudan,Rhodamine B and Carmine in the chili seasonings by HPLC was studied. The sample was extracted in a convenient way,separated in C18 column,and tested by DAD detector. Results showed chromatographic peaks were independent and complete,separated with good linearity and the baseline flat in the 6 kinds of industrial detection.The recovery rate was 90. 7% ~ 104% while the relative standard deviation was between 1. 35% and 4. 75%. Therefore,thanks to the simplicity,rapidity and accuracy,this method will be suitable for the determination in 6 kinds of industrial detection of chili and its products at the same time.