河朔荛花根中抑菌活性成分研究

Studies on antifungal components in the roots of Wikstroemia chamaedaphne Meisn.

在活性追踪指导下,采用多种分离技术从河朔荛花根甲醇提取物中分离得到12个化合物,经核磁共振波谱、质谱等技术并结合相关文献确定其结构分别为1-己酸-2,3-硬脂酸甘油三酯(1)、β-谷甾醇(2)、月桂醇(3)、顺-十八碳-9-烯酸(4)、(2E)-庚基-3-(3, 4-二羟基苯基)丙烯酸酯(5)、(–)-pluviatolide(6)、1β-hydroxy-10β-H-guaia-4,11-dien-3-one(7)、异狼毒素(8)、新狼毒素B(9)、瑞香酚(10)、单棕榈酸甘油酯(11)和(+)-去甲络石甙元(12)。其中,化合物3、4和6～12均为首次从河朔荛花中分离得到。抑菌活性测定结果表明:异狼毒素(8)和新狼毒素B(9)对马铃薯晚疫病菌Phytophthora infestans菌丝生长和孢子萌发均表现出明显的抑制作用,其中对菌丝生长的EC50值分别为68.4μg/mL和44.9μg/mL,对孢子萌发的EC50值分别为25.1μg/mL和14.0μg/mL。

In this study,12 compounds were isolated from the methanol extract of Wikstroemia chamedaphne Meisn.using a variety of separation techniques.Based on the NMR,MS and the data in literatures,they were identified as 1-hexanoic acid-2,3-stearic acid triglyceride(1),β-sitosterol(2),dodecanol(3),cis-octadeca-9-enoic acid(4),(2 E)-heptyl-3-(3,4-dihydroxyphenyl)acrylate(5),(–)-pluviatolide(6),1β-hydroxy-10β-H-guaia-4,11-dien-3-one(7),isochamaejasmin(8),neochamaejasmin B(9),stelleranol(10),glyceryl monopalmitate(11)and(+)-nortrachelogenin(12).Among them,compounds 3,4 and 6-12 were isolated from this plant for the first time.The results of antifungal activity evaluation indicated that isochamaejasmin(8)and neochamaejasmin B(9)showed significant inhibitory effects on the mycelial growth and the spore germination of Phytophthora infestans.The EC50 values of 8 and 9 were 68.4μg/mL and 44.9μg/mL for the growth of P.infestans,respectively.And the EC50 values of 8 and 9 were 25.1μg/mL and 14.0μg/mL,respectively,for the spore germination of P.infestans.