长翅型与短翅型褐飞虱对杀虫剂的敏感性比较

Comparative study on the susceptibility of macropterous and brachypterous Nilaparvata lugens to insecticides

采用稻苗浸渍法测定了长、短翅型褐飞虱对烯啶虫胺、环氧虫啶、呋虫胺、噻虫嗪、噻虫胺、吡虫啉、毒死蜱、敌敌畏、噻嗪酮、异丙威、吡蚜酮和醚菊酯的敏感性,并对其体内解毒酶活力进行了比较分析。结果表明:长翅型与短翅型褐飞虱若虫对新烟碱类杀虫剂呋虫胺、噻虫嗪、噻虫胺和吡虫啉的敏感性存在显著差异,长翅型比短翅型更敏感;相反,对于有机磷类杀虫剂毒死蜱,短翅型褐飞虱则更敏感;2种生物型对烯啶虫胺、环氧虫啶、敌敌畏、噻嗪酮、异丙威、吡蚜酮和醚菊酯的敏感性无显著差异。解毒酶相对比活力测定结果表明,长翅型褐飞虱若虫酯酶比活力显著高于短翅型,细胞色素P450单加氧酶比活力显著低于短翅型,而谷胱甘肽S-转移酶比活力无显著性差异。本研究结果可为褐飞虱的有效防控提供科学参考。

The susceptibility to twelve commonly used insecticides and detoxification enzyme activity of wing dimorphic Nilaparvata lugens were compared.The rice seedling dipping method was used for the bioassay of nitenpyram,cycloxaprid,dinotefuran,thiamethoxam,clothianidin,imidacloprid,chlorpyrifos,dichlorvos,buprofezin,isoprocarb,pymetrozine and etofenprox.The results showed that macropterous N.lugens was more susceptible to the neonicotinoid insecticides including dinotefuran,thiamethoxam,clothianidin and imidacloprid than that of the brachypterous speices.On the contrary,brachypterous N.lugens was more susceptible to the organophosphorus insecticide chlorpyrifos than the macropterous speices.While no significant difference in the susceptibility to nitenpyram,cycloxaprid,dichlorvos,buprofezin,isoprocarb,pymetrozine and etofenprox was observed between those two biotypes of N.lugens.The results of relative specific activity of the detoxification enzyme assay showed that the specific activity of esterase of macropteous N.lugens was significantly higher than that of the brachypteous speices,while the cytochrome P450 monooxygenase specific activity was significantly lower than that of brachypteous speices.The specific activity of glutathione S-transferase showed no significant difference.These results will be helpful for the chemical controlof the wing dimorphic N.lugens.