野艾蒿中双四氢呋喃类木脂素的提取分离及其抑菌活性

Bistetrahydrofuran lignans isolated from Artemisia lavandulaefolia DC and their antifungal activities

为筛选新型植物源杀菌剂,对野艾蒿Artemisia lavandulaefolia DC.地上组织中的化学成分进行了研究。采用甲醇提取、液-液萃取、柱层析及半制备高效液相色谱等技术从乙酸乙酯浸膏中分离得到6个化合物;利用质谱及核磁共振等波谱技术确定这6个化合物分别是epiashchantin (1)、ashantin (2)、sesartemin (3)、epimagnolin A (4)、epiyangambin (5)和diayangambin (6),均属于双四氢呋喃类木脂素。采用菌丝生长速率法测定了其抑菌活性,结果表明:化合物1和3对番茄早疫病菌Alternaria solani的菌丝生长表现出明显抑制作用,EC50值分别为14.3和11.0μg/mL;化合物3、5和6对玉米纹枯病菌Rhizoctonia solani的菌丝生长也表现出抑制作用,EC50值分别为17.4、42.6和54.3μg/mL。

In order to discover novel botanical fungicides,the chemical constituents of the aerial parts of Artemisia lavandulaefolia DC.were investigated.The aerial parts were extracted with methanol.By using liquid-liquid extraction,column chromatography,high performance preparative liquid chromatography and other techniques,6 pure compounds were finally isolated from the ethyl acetate phase.The compounds were identified as bistetrahydrofuran lignans,including epiashchantin(1),ashantin(2),sesartemin(3),epimagnolin A(4),epiyangambin(5)and diayangambin(6),according to NMR and MS data.The mycelium growth rate method was used to determine the antifungal activities.The results showed that compound 1 and 3 significantly inhibited the growth of Alternaria solani with the EC50 value of 14.3μg/mL and 11.0μg/mL,respectively.Compound 3,5 and 6 also showed inhibitory effect against the mycelia growth of Rhizoctonia solani with the EC50 value of 17.4,42.6 and54.3μg/mL,respectively.