枳术汤对脾虚便秘小鼠血浆中P物质和血管活性肽的影响

Effect of Zhi-zhu Decoction on substance P and vasoactive intestine peptide of plasma in spleen deficiency constipation mice

目的:通过观察脾虚便秘小鼠血浆中P物质(substance P,SP)、血管活性肽(vasoactive intestine peptide,VIP)表达的变化,探讨枳术汤对脾虚便秘小鼠胃肠动力靶向调控的机制。方法:采用脾虚泄泻、饥饱失常以及燥结便秘等复合因素造模方法制作脾虚便秘模型;造模组分别予以枳术汤、便秘通口服液、蒸馏水干预;用放射免疫方法观察SP和VIP表达情况。结果:模型组和蒸馏水组的SP和VIP的表达均较正常对照组减少(P【0.01),经治疗后,枳术汤大、中剂量组恢复接近至正常水平,与模型组和蒸馏水组比较有显著差异(P【0.01)。结论:枳术汤对脾虚便秘小鼠模型胃肠动力的调控可能与SP和VIP有关。

Objective:To observe the effect of Zhi-zhu Decoction on substance P(SP) and vasoactive intestine peptide (VIP) of plasma in mice with spleen deficiency constipation. Methods: The mice models of spleen deficiency constipation were established by com- pound factors of splenasthenic diarrhea,irregular diet and fluid-consuming constipation,and then treated with Zhi-zhu Decoction, Bian Mi Tong Oral Liquid or distilled water.The expression of SP and VIP were tested by radioimmunoassay. Results:In the groups of mice models and treated with distilled water,the expression of SP and VIP are lower than those in control group(P<0.01),After treat- ment,the expression recover close to normal level in the high and middle dose groups of Zhi-zhu Decoction,there are significant dif- ferences compared with model and distilled water groups (P<0.01). Conclusion:Target regulation mechanisms of Zhi-zhu Decoction on the gastrointestinal motility of spleen deficiency constipation may be relate to SP and VIP.