聚乙二醇胁迫对假俭草谷胱甘肽转硫酶活性的影响

Effects of PEG Stress on Gluathione S-Transferase Activities in the Seedlings of Common Centipede Grass (Eremochloa ophiuroides)

为探明谷胱甘肽转硫酶(GST)在水分胁迫中的作用,分别用聚乙二醇6000模拟干旱处理假俭草不同时间后,测定假俭草植株的质膜电解质渗透率,GST活性,H2O2和还原型谷胱甘肽(GSH)含量.结果表明:PEG胁迫使幼苗H2O2积累,质膜电解质渗透率增加,GSH含量升高;胁迫处理后幼苗中GST活性明显高于对照,在4d内,随着水分胁迫处理时间的延长,GST活性逐渐升高.GST活性与H2O2的含量相关,表明GST不但能清除H2O2引起的有害物质,同时还很可能受H2O2的调节.

To probe into the role of GST in water stress,GST activities in the seedlings of common centipede grass ( Eremochloa ophiuroides ) treated by PEG6000 are investigated.The results show that H 2O 2 and GSH accumulated, and the ratio of electrolyte leakage increased.GST activities in the seedlings treated by PEG are higher than that of CK, and increased gradually with elongation of stress time. The changes of GST activities have a postive correlation with the acculumlation of H 2O 2( r =0.868) ,which suggests that GST may not only clean oxidative products in cell,but also be regulated by H 2O 2.