摘要
目的从细胞分子水平研究抗纤软肝冲剂对肝星状细胞(HSC)胶原降解的影响。方法抗纤软肝冲剂药物血清温育传一代HSC,以生理盐水和秋水仙碱为对照。ELISA法测定细胞上清Ⅰ型胶原,并测定细胞层总蛋白以校正胶原含量;半定量RT-PCR法检测间质胶原酶(MMP1)和金属蛋白酶组织抑制因子-1(TIMP-1)的mRNA表达。结果抗纤软肝冲剂组细胞上清中的Ⅰ型胶原含量明显低于其他两组(P<0.05或P<0.01);明显促进细胞MMP1的mRNA表达(P<0.05或P<0.01),显著抑制细胞TIMP-1的mRNA表达(P<0.05或P<0.01)。结论抗纤软肝冲剂能促进MMP1基因表达,抑制TIMP-1基因表达,从而促进胶原降解,这可能是该方抗肝纤维化的细胞分子学机制之一。
Objective To study the effect of Kangxian Ruangan Granule(KRG)on the collagen degradation in hepatic astrocytes(HA)at the cellular and molecular level.Methods The medicated sera of KRG was used to i ncubate the sub-cultured HA and sodium chloride and c olchicines were taken as controls.T he content of typeⅠcollagen in the cultured su-pernatant was measured with ELISA an d then was corrected by assaying the t otal protein in cell layer.The mRNA e xpres-sions of matrix metalloproteinase -1(MMP1)and tissue inhibitor of metalloproteinase -1(TIMP -1)were analyzed by semiquantitative RT -PCR.Results The content of typeⅠcollagen in the KRG -cultured supern atant was lower than that in the other two groups(P<0.05,P<0.01);KRG obviously promoted mRNA expression of MMP1(P<0.01)and inhibited mRNA expression of TIM P -1(P<0.05,P<0.01).Conclusion KRG can enhance the collagen degra-dation by promoting the gene expression of MMP1and inhibiting the gene ex pression of MMP1,which may be the cellular and molecular mechanism of its antif ibrotic action.
出处
《中药新药与临床药理》
CAS
CSCD
2003年第3期158-161,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
