新的血管生成抑制肽(手性制剂)抗肿瘤血管生成的实验研究

Experimental study on tumor angiogenesis inhibition effect of new angiogenesis peptide (chiral preparation)

目的 观察一种新的血管生成抑制肽对肿瘤的抑制作用 ,并探讨其作用机制。方法 体外药效实验 ,采用 MTT法及血管内皮细胞迁移法 ,体内用 L ewis肺癌瘤株皮下接种 C57BL/ 6N小鼠 ,观察皮下移植瘤生长情况。结果 新的血管生成抑制肽对血管内皮细胞增殖、迁移有明显的抑制作用 ,在体内使 Lewis肺癌皮下移植瘤体积明显缩小 ,毛细血管稀少。结论 新的血管生成抑制肽能明显抑制 L ewis的肿瘤生长 ,其机制可能与抑制肿瘤血管生成有关。

Objective To observe the effect of a new angiogenesis peptide on tumor and possible mechanism.Methods The MTT assay and endothelial cell migration methods were used for the test of drug-effect in vitro and subcutaneous inoculation C57BL/6N with Lewis carcinoma of lung oncocyte for vivo growth condition.Results There is the obvious inhibitional effect on propagation endothelial cell propagation and migration, and obvious volume miniture and capillary blood vessel rarus were observed at Lewis carcinoma of lung subcutaneous oncocyte in vivo.Conclusions The new angiogenesis peptide possible obvious inhibites Lewis tumor growth ,the mechanism may correlate to inhibition on tumor angiogenesis.