丹参酮对海马内注射β-淀粉样肽后大鼠学习记忆障碍的影响

Influence of tanshinone on learning and memory impairment induced by amyloid β-peptide1-40 in rats

目的 观察丹参酮对 β-淀粉样肽 1～ 40片段 (Aβ1～ 40 )诱导大鼠学习记忆功能障碍的改善作用。方法 应用凝聚态 Aβ1～ 40在大鼠海马内注射以建立动物模型 ;采用明暗箱被动回避法、穿梭法测试学习记忆功能 ;采用 HE染色、结晶紫染色和 Nissel染色观察病理组织。结果 大鼠双侧海马内注射 Aβ1～ 40 (2 0 μg)后 ,经行为学测试 ,其学习记忆功能明显障碍 ;HE染色、Nissel染色发现海马受损区内神经细胞数量明显减少 ,胶质细胞呈反应性增多 ;结晶紫染色显示海马内大量淀粉样蛋白沉积。丹参酮 (1 0 0、50、2 5 mg/kg)连续灌胃 1 4 d,处理后分别对上述改变有不同程度的抑制作用 ,其中尤以 50 mg/kg剂量组效果最好。结论 丹参酮对 Aβ1～ 40诱导大鼠的学习记忆功能障碍具有显著改善作用。

Objective To investigate improvement of tanshinone on learning and memory impairment induced by amyloid β-peptide1-40(Aβ1-40) in rats. Methods 10 μg incubated Aβ1-40 was injected bilaterally into the dorsal blade of the dentate gyrus in the hippocampus of each rat. Two- compartment step-through passive avoidance test and shuttle-box active avoidance response test were used together to determine effects of Aβ1～40 and that of tanshinone on learning and memory. Pathological changes were detected with the method of HE, Crystal violet,and Nissel staining. Results Learning and memory dysfunction was detected by the behavioural test after Aβ1～40 injection . Loss of neurons and glial reaction in or around the injection sites were shown by HE and Nissel staining . Aβdepositions were also found in the hippocampus using Crystal violet staining . With tanshinone administration (100?50?25 mg/kg, ig ×14 d) , the changes mentioned above could be inhibited respectively. Conclusions Tanshinone improved the impaired learning and memory induced by Aβ1～40 in rats.