下丘脑外侧区对大鼠胃缺血-再灌注损伤调控的神经机制

The neuroregulatory mechanism of lateral hypothalamic area on gastric ischemia-reperfusion injury in rats

目的 :观察电刺激下丘脑外侧区 (LHA)对胃缺血 -再灌注损伤 (GI -RI)的影响 ,并初步分析其可能的神经机制。方法 :用电和化学刺激、电损毁神经核团及切断外周神经等方法 ,观察LHA对大鼠胃缺血 30min ,再灌注 6 0min所致损伤的影响并分析背侧迷走复合体 (DVC)、迷走神经和交感神经在这一效应中的作用。结果 :①电刺激LHA或LHA内注射L -谷氨酸 (L -Glu) ,GI-RI均显著加重 ;②电损毁双侧LHA则减轻GI-RI;③损毁双侧DVC能取消电刺激LHA对GI -RI的加重 ;④分别切断膈下迷走神经和切除腹腔交感神经节后再电刺激LHA ,GI-RI则减轻。结论 :LHA是对GI-RI具有加重作用的特异性中枢部位 ,DVC以及迷走神经。

AIM: To determine the effect of electrical stimulation of lateral hypothalamic area(LHA) on gastric ischemia-reperfusion injury(GI-RI) in rats and to analyse its possible neuroregulatory mechanisms. METHODS: The methods of electrical stimulation(ES), chemical stimulation, electrolytic lesion(EL) and denervation were used to investigate the effect of LHA ES on gastric mucosal injury in rats subjected to 30 min gastric ischemia followed by 60 min reperfusion and to analyse the role of dorsal vagal complex(DVC), vagus and sympathetic nerve in this effect. RESULTS: ①Electrical stimulation of LHA and microinjection of L-glutamic acid into LHA obviously aggravated GI-RI; ②Electrolytic lesion of the LHA attenuated the GI-RI;③DVC lesion eliminated the effect of electrical stimulation of LHA on GI-RI; ④Vagotomy or sympathectomy eliminated the effect of electrical stimulation of LHA on GI-RI too.CONCLUSION: These results indicate that the LHA is a specific area in the CNS for exerting aggravative effects on the GI-RI; the DVC, vagus and sympathetic nerve may be involved in regulatory effects of LHA on GI-RI.