缺血预处理前加用腺苷对未成熟兔心肌再灌注损伤的影响

Effect of adenosine infusion before ischemic preconditioning on immature myocardial reperfusion injury in neonatal rabbits

目的和方法 :建立 3- 4周龄幼兔离体心脏左心作功模型 ,心脏缺血期间均间断灌注心脏停搏液 ,研究缺血预处理前加用腺苷对未成熟兔再灌注心肌的保护作用并探讨其机制。结果 :再灌注期 ,缺血预处理前加用腺苷的心脏的左室收缩压 (LVSP)、左室舒张末压 (LVEDP)、左室内压上升最大速率 ( +dp/dtmax)和左室内压下降最大速率 ( -dp/dtmax)恢复最佳 ,肌酸激酶 (CK)漏出率低 ,心肌组织丙二醛 (MDA)含量低、超氧化物歧化酶 (SOD)活性高 ,心肌组织三磷酸腺苷和二磷酸腺苷 (ATP +ADP)含量高 ;心肌含水量 (MWC)低。结论

AIM and METHODS: To investigate the cardioprotective effect of adenosine infusion before ischemic preconditioning on immature myocardial reperfusion injury in rabbit heart. Isolated perfused working heart model were performed, all hearts were subjected to 2-hour global hypothermic ischemia and received intermittent cold cardioplegia perfusion. RESULTS:During reperfusion, the recovery of left ventricular systolic pressure, left ventricular end-diastolic pressure, +d p /d t max, and -d p /d t max of hearts received adenosine infusion before ischemic preconditioning were significantly improved, myocardial adenosine triphosphate and adenosine diphosphate content and superoxide dismutase activity were higher, the leakage of myocardial creatine kinase and the malondialdehyde content were lower, and myocardial water content was obviously less. CONCLUSION: These results suggest adenosine infusion before ischemic preconditioning enhances cardioprotection of ischemic preconditioning against immature myocardial reperfusion injury in the rabbit heart.