抗CD59对CVF激活人血小板的影响

Effect of anti-CD59 on CVF-induced platelet activation

目的 :研究补体的激活对人血小板的作用及其抗CD5 9对该作用的影响。方法 :应用眼镜蛇毒因子(CVF)制成补体活化血小板的模型 ,通过测定健康成年男性血小板的聚集及释放曲线 ,观察抗CD5 9应用前后补体对血小板变形、聚集及释放功能的影响。结果 :CVF能诱导健康成年男性的血小板显著、持久地变形和释放 ,但不能诱导血小板聚集。当CVF浓度在 12 .5 - 5 0 0g/L范围内 ,血小板的最大变形幅度与CVF浓度的对数呈线性相关 (r=0 970 ,P <0 .0 1,n =36 )。抗CD5 9能增强健康成年男性血小板的最大变形幅度 ,并与剂量相关 ,其血小板最大变形幅度的最大增加值为空白对照的 1.36倍 (P <0 .0 1)。同时抗CD5 9也促进补体诱导的血小板ATP分泌增多。结论 :活化补体后能诱导健康成年男性血小板发生变形、释放 ,但不诱导血小板的聚集 ,且抗CD5

AIM: To study the reactions of human platelet to active complement and the effects of anti-CD59 on human platelet activation induced by complement. METHODS: By applying CVF to activate complement, the platelet aggregation and release reactions induced by activated complement with or without appling anti-CD59 with different doses to block the complement modulative protein CD59 in healthy individuals, were observed. RESULTS: CVF induced platelet release and significant and lasting metamorphosis in healthy individuals, but platelet aggregation was not observed. CVF-induced platelet metamorphosis showed positive linear correlation to lg concentration of CVF (r=0 970. P<0.01. n=36) . Anti-CD59 enhanced CVF-induced platelet shape change with a dose-dependent manner. The max enhancive ratio of platelet shape change was 1.36( P <0.01). Anti-CD59 enhanced platelet ATP release induces by CVF. CONCLUSION: Complement activated by CVF induces significant and lasting platelet metamorphosis and release reaction, but dose not induce platelet aggregation in healthy adult males. Anti-CD59 promotes the platelet reactions induced by active complement.