三七皂苷单体Rg1对D-半乳糖模型鼠学习记忆和免疫功能的影响

Effect of Rg1 on learning-memory behavior and immune function in mice treated with D-galactose

目的 :探讨三七皂苷单体 Rg1对 D-半乳糖 ( D- gal)所致衰老模型鼠学习记忆行为和免疫功能的影响。方法 :Balb/c小鼠随机分为 4组 :衰老模型组、正常对照组、实验 组和实验 组。应用跳台和 Y-型迷路方法测定 D-半乳糖衰老模型鼠学习记忆行为 ;采用 3 H- Td R掺入法测定小鼠胸腺、脾细胞的增殖能力 ;生物活性检测法测定 IL- 2和 TNF- α水平的变化。结果 :三七皂苷单体 Rg1能明显提高衰老模型鼠对电刺激的逃避能力 ,改善 D- gal所致学习记忆能力下降。同时三七皂苷Rg1能明显增强衰老模型鼠胸腺和脾细胞增殖能力 ,对 IL- 2水平有所提高 ,血清 TNF- α的细胞毒作用较模型组降低。结论 :三七皂苷单体 Rg1能改善衰老模型鼠学习记忆能力并调节免疫功能。

Objective: To investigate the role of P. pseudo ginsengvar.notoginseng Rg1 on the learning memory and immune function in D galactose induced mouse aging model. Methods:Balb/c mice were randomly divided into four groups,including D glactouse model group ,normal control group , experiment group I and experiment group Ⅱ. Stepdown and Y maze were performed to evaluate their learning and memory function , 3H thymidine incorporation( 3H TdR) were employed to determine the potency of thymus and spleen lymphocyte proliferation. The level of IL 2 and TNF α were measured by bioassay method. Results: Compared with the D galactose induced mouse model, Rg1 could improve significantly the ability of escaping electrical stimulation, increase thymus and spleen lymphocyte proliferation, alleviate the change of IL 2 and TNF α level. Conclusion:The results suggested that Rg1 may be beneficial in improving the aging process and regulating of immune function.