吸烟烟气对鼠肺细胞膜的损伤和茶多酚的保护作用

TOXICOLOGICAL EFFECTS OF GAS-PHASE CIGARETTESMOKE ON RAT LUNG CELLS AND PROTECTION EFFECTS OF GREEN TEA POLYPHENOLS IN THE SYSTEM

本文以香烟气相物质作用鼠肺细胞膜为模型,用脂肪酸自旋标记物5-DOXYL和16DOXYL分别研究膜浅层和深层的动态性质受气相烟的影响,并用紫外可见分光光度法研究气相烟对膜脂的作用。结果发现,在实验的气相烟流量下,香烟气相物质能引发鼠肺细胞膜的脂质过氧化,并且使膜浅层的流动性增大。但对膜深层的动态性质没有明显的改变,如果在鼠肺细胞中预先加入粗晶态或粉态茶多酚。则肺细胞的过氧化和膜的动态性质改变受到抑制,而且这种抑制作用与茶多酚浓度呈量效关系,而茶多酚本身对膜浅层无明显作用,但对膜深层的流动性有一定影响,而且两种茶多酚的作用相似。

Toxicological effects of gas-phase cigarette smoke (GPCS) on rat lung cells (RLC) were investigated by using ESR spin label and Ultraviolet-Vision techniques.5-doxyl and 16-doxyl stearic acid were used as spin labels. It was found that GPCS was able to induce lipid peroxidation of RLC and increase the membrane fluidity in shallow-layer, while had little effect on the deep-layer. If powder and crude-crystal green tea polyphenols (GTP) were added into the suspension of RLC before GPCS treatment, it was discovered that lipid peroxidation of RLC declined and the membrane fluidity recovered with increasing the concentration of GTP. GTP itself had little effect on the shallow-layer of RLC membrane and had a little influence on the deep-layer.