百合甾体皂苷元的气-质联用分析及其结构鉴定

GC/MS Analysis of Steroidal Sapogenins from Bulbs of Lilium brownii var.colchesteri and Their Identification

运用气相色谱 质谱联用仪(GC/MS)对来自百合Liliumbrowniivar.colchesteri中的2种甾体皂苷元进行了直接分析,并根据其GC/MS、红外光谱、紫外光谱和核磁共振碳谱(13C NMR)等数据对其结构进行了鉴定.结果表明:气 质联用仪(GC/MS)直接分析甾体皂苷元快速、方便,不仅需要样品量少,而且能同时获得样品的色谱图和质谱图,适合于大量试样的分析.2种百合皂苷元的光谱数据经分析,并与文献标准对照,鉴定百合皂苷元1为薯蓣皂苷元(diosgenin),百合皂苷元2为提果皂苷元(tigogenin).

In this article, two kinds of steroidal sapogenins from bulbs of Lilium Brownii Var.colchesteri were analyzed by gas chromatographymass spectrometry (GC/MS) and their identification were conducted by the data of EI/MS, IR, UV and 13C NMR. The results showed that direct analysis of steroidal sapogenins by GC/MS is simple and convenient. The method only needs very little sample to obtain both the sample's chromatograph and its EI/MS spectrum. Thus it was particularly suitable for analysis of a large amount of steroidal sapogenins samples. Spectra data of the two steroidal sapogenins from bulbs of Lilium brownii var.colchesteri were analyzed and compared with the literature standards. Their identification were conducted. One is SpirostanEn3Ol, 3. Beta, 5. Alpha, 25R(diosgenin), and the other is Spirostan3Ol, 3.Beta.,5.Alpha.,25R(tigogenin).