耐热芽孢杆菌XJT9503高温中性蛋白酶的中试发酵工艺

Study on the Pilot Fermentation Technology of the XJT9503 Thermostable Neutral Protease

对耐热芽孢杆菌(Thermophilicbacillus)XJT9503保藏、菌种活化、种子培养基和发酵培养基及7,25,250,3000L发酵罐的中试发酵工艺参数进行了研究,确定了耐热芽孢杆菌XJT9503高温中性蛋白酶的中试发酵工艺.在3000L发酵罐上控制罐温为46℃、转速180r/min、通气量4L/(L·min),培养18h后发酵酶活最高达13240U/mL.

In order to industrialize the XJT9503 thermostable neutral protease, the strain preservation and activation methods, seed medium, fermentation medium and fementation technologies under various scales(7, 25, 250, 3 000 L) were studied. The optimal pilot fermentation conditions were determined. A maximum enzyme productivity of 13 420 U/mL in a 3 000 L fementor was obtained at 18 h under the fermentation conditions of, 46 ℃, 180 r/min, and 4 L/(L·min).