冠心病及2型糖尿病合并冠心病与对氧磷酯酶192Gln/Arg基因多态性的相关性研究

Relation between paraoxonase 192Gln/Arg gene polymorphism and coronary heart disease in and not in type 2 diabetes mellitus

为探讨冠心病 (CHD)及 2型糖尿病 (DM )合并CHD与对氧磷酯酶 (PON1) 192Gln/Arg基因多态性的关系 ,采用多聚酶链反应 限制性片段长度多态性 (PCR RFLP)法检测了 10 4例查体健康者 (对照组 )和 76例CHD、96例 2型DM合并CHD患者的PON1 192位点的多态基因型 ;同时测量其体重指数 (BMI) ,检测总胆固醇(TC)、甘油三酯 (TG)、高密度脂蛋白胆固醇 (HDL C)、低密度脂蛋白胆固醇 (LDL C)、载脂蛋白AⅠ (ApoAⅠ )、载脂蛋白B(ApoB)。结果显示 ,PON1 192位点QQ、QR、RR基因型及Q、R等位基因频率在 2型DM合并CHD组与对照组比较均有显著性差异 (P <0 0 5、<0 0 1) ;但CHD组与对照组比较无显著性差异 (P >0 0 5 )。中国人与白种人PON1 192位点基因型及等位基因频率比较有显著性差异 (P <0 0 1)。含R等位基因的基因型QR、RR是 2型DM合并CHD的独立危险因素。提示PON1 192Gln/Arg基因多态性与 2型DM合并CHD有相关性 。

In order to investigate the relation between paraoxonase (PON1) 192Gln/Arg gene polymorphism and coronary heart disease (CHD) in and not in type 2 diabetes mellitus(DM),polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was used to measure the PON1-192Gln/Arg genotype in 104 healthy subjects,76 patients with CHD and 96 type 2 DM patients with CHD.Meanwhile,body mass index (BMI),and serum lipid levels of all subjects were determined.Results showed that the frequency of the PON1-192Gln/Arg genotype and the allele had significant difference between type 2 DM patients with CHD and healthy subjects(P<0.05,0.01),but that had no difference between patients with CHD and healthy subjects (P>0.05).The frequency of the PON1-192Gln/Arg genotype and the allele had significant difference between Chinese and the White(P<0.01).The genotypes carried R allele were independent risk factors of type 2 DM with CHD.These suggest that PON1-192Gln/Arg gene polymorphism is associated with CHD in type 2 DM.