高效液相色谱电化学检测法测定猪组织中的克伦特罗

Determination of Clenbuterol in Pig Tissues by High Performance Liquid Chromatography with Electrochemical Detection

建立了猪组织中克伦特罗的高效液相色谱 电化学检测方法。色谱柱为DiscoveryC18柱(5μm,4 6mmi.d.×250mm);流动相为1.7mmol/L氯化锂 甲酸 甲醇(体积比为65∶1∶34)混合溶液,流速为1 0mL/min;柱温为40 0℃;电化学检测器工作电位为1 25V。猪组织中克伦特罗含量为0 5～500ng/g时,克伦特罗的含量与其峰面积之间存在良好的线性关系(r>0 99),在组织中按0 5,50,500ng/g3个添加水平做克伦特罗的回收率试验,其回收率为75 8%～87 1%(n=4)。该方法重现性好,灵敏度高,简便,可用于猪的肌肉、肺脏、肝脏、肾脏及脂肪组织中克伦特罗残留的检测。

A convenient and reliable high performance liquid chromatographic method (HPLC)with electrochemical detection was developed for the deterimination of clenbuterol in pig tissues. The tissue samples were homogenized and the drug was extracted by liquidliquid partitioning with an organic solution. Separation conditions are as follows: a Discovery C18 column (5 μm ,46 mm i.d.×250 mm), 1.7 mmol/L LiClformic acidmethanol (65∶1∶34, v/v) solution as mobile phase, flow rate 10 mL/min and column temperature 400 ℃. The working potential of the electrochemical detector was 125 V. The calibration curves were linear (r>099) for clenbuterol concentrations of 05-500 ng/g in pig tissues. The recoveries ranged from 758% to 871% at three spike levels of 05, 50 and 500 ng/g. The limit of quantitation (LOQ) was 05 ng/g in pig tissues such as muscle, lung, liver, kidney and adipose. The method showed good reproducibility, high sensitivity, and fast speed. 