真核基因选择性剪接机理的初步研究

Alternative splicing mechanism for eukaryotic gene

真核基因表达调控是生命科学研究的前沿、热点。RNA的选择性剪接在真核基因表达调控中起着十分重要的作用。该文从已有数据出发 ,对真核基因的剪接机制以及选择性剪接的机制进行了初步的研究。结果表明 ,在真核基因的剪接过程中 ,可能存在一种“粗定位—细定位”的过程 ,即在剪接过程中首先有一粗略的定位过程 ,根据序列的嘌呤、嘧啶浓度特征寻找出剪接位点的大致位置 ;然后在这一基础上 ,根据几个保守碱基所提供的信息找到准确的剪接位点。这一结果对于进一步研究真核基因的剪接机制 ,特别是选择性剪接发生的机理有很大的启发。

The control of eukaryotic gene expression is currently a key focal point of life science research. Research has shown that mRNA affects gene regulation and expression, so splicing, especially alternative splicing of RNA, will affect the control of eukaryotic gene expression. Existing data was used for an initial study of the splicing and alternative splicing mechanism of eukaryotic genes. The results showed that approximate and exact orientations could be identified for the splicing of eukaryotic genes. The approximate orientation process located approximate splice site positions by analyzing the pyrimidine (or purine) concentration of nucleic acid sequences. The exact sites were then located using information for several conserved purines or pyrimidines using information from the first step. The methodology can be used for further research on the splicing mechanism, especially alternative splicing of eukaryotic genes.