摘要
DNA加合物是具有亲电性化学致癌物与DNA形成共价相联的化合物,是化学致癌物损伤DNA的主要形式。
Reaction of DNA-styrene oxide produced six adducts as determined by P1-nuclease mediatedi enhancemet of sensitivity of 32P-post-labeling in vitro. In this procedure, nuclease P1 cleaves deoxyribo-nucleoside 3'-monophosphates of normal nucleotides to deoxy-ribo-nucleosides which do not serve as substrate for polynucleotide ki-nase,while most adduct nucleotides were found to be totally or partially resistant to the 3'-dephosphorylating action of nuclease P1. Five adducts were detected quantitatively with a 150 to 800 fold increase in 32P-count rates as compared with the standard procedure (without P1 nuclease).
出处
《环境科学学报》
CAS
CSSCI
CSCD
北大核心
1992年第2期254-259,共6页
Acta Scientiae Circumstantiae
