对硫磷的人工抗原合成与鉴定

SYNTHESIS AND IDENTIFICATION OF ANTIGENIC CONJUGATE AGAINST PARATHION

为防治病虫草害,确保农业丰收,每年有大量农药化学品施入环境中,从而使环境监测任务日趋繁重.气相色谱、液相色谱这些传统的农药残留检测手段,因其仪器昂贵,前处理过程复杂等难以满足快速监测需要.酶联免疫吸附分析(Enzyme Linked ImmunoSorbent Assay简称ELISA)技术,具有设备简单、成本低廉、专一性强、灵敏度高、样品预处理简化、适于同时检测大量样品等优点.近年来已发展成为一种简单、灵敏、快速的农药残留测试技术而应用于环境监测中.

A method of synthesis of antigen by reducing parathion into amino-para-thion and directly conjugating to a carrier protein BSA via diazo coupling was presented. Microphosphorus determination of the conjugate revealed 9±1 redu-ced-parathion residues per BSA molecule.