双曲孢菌(Nakataea sigmoidea Hara)生物学特性初步研究

Biological Characters of Nakataea sigmoidea Hara

为了研究双曲孢菌 ( N akataea sigmoidea Hara)在不同环境及营养条件下的产孢特性 ,分别在黑暗条件和光照条件下 ,通过改变环境因子 ( p H值、温度、固体培养、液体培养 )和营养因子 (碳源、氮源、天然、合成 )来进行产孢培养试验。结果表明 ,双曲孢菌在黑暗条件下 ,不同环境因子和不同营养因子下均不能产生分生孢子 ;在自然光条件下 ,1.5 %水琼脂、 Czapek培养基上 ,菌核和菌丝均能产孢。碳氮比对菌核萌发产生分生孢子有显著影响 ( P <0 .0 1) ,以碳氮比为 10 :1时 ,产孢率最高 ,达 5 4 .2 3%。紫外灯照射 10

The experiment of conidia production are conducted in the conditions of natural light and dark in order to research into the growth behaviour of conidia of Nakataea sigmoidea Hara The facts of pH value,temperature,substrates (liquid and solid),C source,N source,inartificial and composite nutriments are considered In the darkness,N sigmoidea Hara could not produce conidia in any surroundings or substrates In the nature light,sclerotia and mycelium could produce conidia in the substrates of 1 5% water agar and Czapek The ratio of C/N has significant effect on the production of conidia from sclerotia(P<0 01) The production rate of conidia gets to the highest (54 23%) when the ratio of C/N is 10∶1 Ten minutes of ultraviolet treatment could stimulate the production of conidia