自体细胞因子诱导的杀伤细胞治疗急性B淋巴细胞白血病22例临床观察

Clinical observe the effects of cytokine induced killers on 22 cases with B-acute lympha leukemia

目的 :观察自体细胞因子诱导的杀伤细胞 (cytokineinducedkiller,简称CIK)治疗急性B淋巴细胞白血病 (B -ALL)的效果。方法 :用血细胞分离机采集患者的单个核细胞 (PBMNC) ,再用抗CD3单抗 (MabCD3)、白细胞介素 2 (IL2 )、干扰素γ(IFNγ)培养 10d ,经洗涤后分两次回输给患者。结果 :2 2例接受CIK治疗的B -ALL完全缓解率 (CCR)为 78% ,中位生存期为 2 4月。其中 7例骨髓存在残留异常基因或异常染色体的患者经CIK治疗 2 - 3疗程后有 6例消失。结论 :自体细胞因子诱导的杀伤细胞 (CIK)治疗急性B淋巴细胞白血病 (B -ALL)疗效显著 ,可清除微小残留白血病 。

Objective: To study the effects of cytokine induced killers treat for 22 cases with B-acute lymphemia leukemia(B-ALL) .Methods:The patients peripheral blood mononuclear cells were collected by blood cell separator,and cultured in the medium containing monoclonal antibody against CD3,interleukine2 and interferonγ for 10 days.finally washed twice and infused back to the patients.Results:22 cases who received the threat of CIKs was 78% with continuous complete remission(CCR),the medium period 24 months;the residual abnormal chromosome or gene 6 cases disappeared from 7 cases after the treat of CIKs.Conclusion:CIK cells can clear out minimal residual leukemia disease(MRD),prevent relapse.