摘要
大鼠肝脏经匀浆、硫酸铵盐析和DEAE-纤维素柱层析,得到部分纯化的蛋白激酶C。将此酶制剂在体外与藻酸双酯钠孵育,藻酸双酯钠对蛋白激酶C活性呈剂量依赖性的抑制作用。
Protein kinase C was partially purified from rat liver by homogenization, ammonium sulfate fractionation and DEAE-cellulose chromatography.After the enzyme was incubated with polysaccharide sulphate, its activity was dose-dependently inhibited by polysaecharide sulphate.
出处
《基础医学与临床》
CSCD
1992年第1期26-28,共3页
Basic and Clinical Medicine
基金
国家自然科学基金
关键词
蛋白激酸C
澡酸双酯钠
protein kinase C polysaccharide sulphate
