应用鸡毒霉原体PCR试剂盒对人工感染鸡的检测

Detection of MG from experimental infected chicken by MG-PCR kit

应用鸡毒霉原体 (MG)聚合酶链式反应 (PCR)试剂盒对人工感染鸡的样品进行检测 ,结果人工感染后第1～ 2 4天 ,所采集喉头及腭裂处粘液、喉气管组织、肺组织均可检出 MG,阳性检出率要比传统分离鉴定方法高 ,试验结果表明了 MG PCR试剂盒对 MG的检测不仅特异、敏感、快速 ,而且操作简便 ,适合在临床上推广应用。

From the first day to 24th day after inoculation, MG could be detected in the throat swabs, the tissues of tracheas and lungs of chicken infected with the MGPCR kit. The positive rate of detection was higher by the kit than by traditional pathogen isolation. The results indicated MGPCR kit is not only specific, sensitive and rapid, but also convenient for diagnosing MG.