姜黄素体外增敏抗肿瘤药物作用

Effect of curcumin on promoting sensitivity for amtitumor drug in vitro

目的 探讨姜黄素与抗癌药物长春新碱、阿霉素合用对KB及KBV2 0 0 细胞的体外杀伤作用。方法 采用MIT法测定药物的体外杀伤作用 ,用荧光分光光度法进行细胞内阿霉素蓄积测定。结果 姜黄素与长春新碱、阿霉素合用 ,在KB及KBV2 0 0细胞中均有增敏作用。蓄积实验说明 ,在KBV2 0 0 细胞 ,其增敏作用与增加细胞内阿霉素蓄积有关 ;而在KB细胞中的增敏作用与细胞内阿霉素蓄积量无关。结论 姜黄素通过不同机理增敏抗癌药对敏感细胞KB及其耐药细胞KBV2 0 0

OBJECTIVE To investigate antitumor effects of curcumin(Cur) combined with Vincristine(VCR) or Adriamycin(ADR) on KB and KB v200 cell lines in vitro. METHODS The antitumor effects of the drugs on tumor cell lines in vitro were determined by MTT method.Cellular ADR accumulation was measured by fluorescence spectrophotometry.RESULTS Cur combining with VCR or ADR produced sensitive effect on KB and KB V200 cells. The cellular ADR accumulation was markedly increased at the presence of Cur in MDR KB v200 cells,but the sensitized effect was none concering with cellular ADR accumulation in sensitive KB cells.CONCLUSION Cur can sensitize effect of antitumor drug by different mechanisms in sensitive KB cell line and MDR KB v200 cell line.