20号柴油低浓度长期暴露对鲫鱼肝脏抗氧化防御系统的影响

Effects of No.20 Diesel on Antioxidant Defenses in Liver of Carassius auratus Under a Long-Term Exposure and Low-Concentration

选择太湖石油烃污染物20号柴油和当地经济鱼类鲫鱼为材料,在室内模拟条件下,研究了低浓度下柴油的长期(40d)暴污对鲫鱼(Carassiusauratus)幼体肝脏抗氧化系统的影响。结果表明,在实验梯度范围内,油污为0.005mg·L-1时即对鱼体肝脏超氧化物歧化酶(SOD)活性表现出显著的诱导作用;而当暴污浓度达到0.01mg·L-1时,过氧化氢酶(CAT)活性、谷胱甘肽过氧化物酶(Se-GPx)活性和还原型谷胱甘肽(GSH)含量均明显被诱导;本实验中,谷胱甘肽转硫酶(GST)的活性总的来说表现为被抑制。CAT、Se-GPx、GSH,尤其是SOD,很有前途成为水生生态系统中石油污染的生物监测指标。

Under indoor simulate condition, No. 20 diesel oil and carassius auratus are chosen as materials to study effects of long- term exposure of trace diesel oil on antioxidant system of larval fishes. The results showed that within the experimental gradient, when oil pollution was at only 0.005 mg· L- 1 , the activity of superoxide dismutase (SOD) was induced significantly. When oil pollution reached 0.01 mg· L- 1, the activities of Catalase (CAT), sedium- dependent glutathione peroxidase (Se- GPx) and the content of reduced glutathione (GSH) were induced apparently; while in this experiment, the activity of glutathione S- transferase (GST) was inhibited in the mass. CAT, Se- GPx, GSH, especially SOD, probably played as oil pollution biomarkers in Taihu ecosystem.