铁离子致创伤后癫痫大鼠模型海马胶质纤维酸性蛋白的动态表达

Glial Fibrillary Acidic Protein Expression of Hippocampal Astrocytes in Post-Traumatic Epilepsy Rat Model

目的:观察创伤后癫痫大鼠模型海马区胶质纤维酸性蛋白的动态表达,探讨反应性胶质细胞增生在创伤后癫痫发病机制中的作用。方法:应用铁离子颅内注射制作创伤后癫痫模型,采用免疫荧光法染色观察海马各区胶质纤维酸性蛋白的表达。结果:7～15d胶质纤维酸性蛋白反应达高峰,注射侧海马CAl区锥体细胞层神经元大量脱失,30d胶质纤维酸性蛋白仍维持较高水平。结论:反应性胶质细胞增生可能与神经无异常放电环路形成有关,最终诱发创伤后癫痫,并成为慢性复发性癫痫的重要病理学基础。

Objective: To observe glial fibrillary acidic protein(GFAP) expression in hippocampal astrocytes in iron-induced epilepsy rat model. Methods:Rats were given an amygdaloid body injection of ferric chloride, and GFAP expression was determined over time using immunofluorexcent methods in hippocampal tissue slices. Results: the intensity of GFAP-staining changed with different time intervals(1-30d)after iron injection, the strongest intensity was on 7-15d, the staining intensity still increased obviously on 30d. Neurons lost largely in stratum pyramid in CA1 of injection lateral. Conclusionlron-induced epileptogenesis involves proliferation and hypertrophy of GFAP-positive astrocytes that may lead to abnormal neural circuits formation and chronic epilepsy recurring.