中药调心方对氧化损伤型类Alzheimer病大鼠线粒体呼吸链酶活性和钙稳态的影响

Effect of HBR on mitochondrial respiratory oxidase and calcium homeostasis of oxidative damaged AD rat model

目的 观察调心方对氧化损伤型类Alzheimer病 (AD)大鼠线粒体呼吸链酶活性和钙稳态的影响。方法 采用二羟延胡索酸 (DHF) +FeCl3+ADP左侧脑室注射法建立氧化损伤型类AD大鼠模型。Morris水迷宫法观察大鼠空间学习记忆能力 ;Clark氧电极法观察脑皮层线粒体呼吸链氧化酶活性 ;原位杂交法观察脑皮层线粒体细胞色素氧化酶Ⅱ型亚基 (COⅡ )mRNA表达 ;Fura 2 /AM 荧光法测定脑皮层和海马Ca2 + 含量以及调心方对AD大鼠上述各项指标变化的影响。结果 与正常对照组比较 ,AD模型大鼠空间学习记忆能力、脑线粒体细胞色素氧化酶活性和皮层COⅡmRNA表达明显降低 ,皮层和海马神经元内Ca2 + 含量显著增高 ;调心方对上述各项指标有不同程度的改善作用。结论 调心方具有改善AD大鼠空间学习记忆能力、脑线粒体细胞色素氧化酶活性、皮层COⅡmRNA表达和钙稳态失衡的作用。

Objective To observe the effect of the Heart Benefiting Recipe(HBR) on the abilities of spatial learning memory.The activities of oxidase in mitochondrial respiratory and calcium homeostasis etc.in brain of the oxidative damaged AD rat model.Methods The rats were injected with the oxygen free radical generation system (DHF-FeCl 3-ADP) in left ventricles of the brain and induced oxidative damaged AD rat model. The spatial learning memory abilities were observed by Morris water maze test. The activities of cytochrome oxidase(CO) were determined using Clark oxygen electrode method.The expression of COⅡ mRNA was observed by in situ hybridization.The contents of cytoplasm Ca 2+ were determined using Fura2/AM- fluorescence method. The regulating effects of HBR on above indices were also studied.Results Compared with the normal group, the abilities of spatial learning memory, the activities of oxidase in mitochondrial respiratory and the expression of COⅡ mRNA were decreased obviously. The contents of calcium in cytoplasm of cortex and hippocampus were increased significantly.HBR, however, could improve the indexes of above mentioned in varying degrees.Conclusions The data demonstrated that the clinical efficacy of HBR on AD may be partly resulted from its improving the abilities of spatial learning memory, the activities of oxidase in mitochondrial respiratory , the expression of COⅡ mRNA and the calcium homeostasis.